[Objective] Exploring the changes and ecological effects of the ecological land in the desert oasis area can provide a decision-making reference for the ecological protection and high-quality development of the Yellow River Basin. [Methods] Taking Yinchuan City, a desert oasis in the upper reaches of the Yellow River, as an example, this study put forward the coefficient revision method of ecological service value (ESV) equivalent factors, established the relationship of ESV equivalent factor coefficients between Yinchuan City and China nationwide, and analyzed the response characteristics of ESV to the spatial and temporal changes of production-living-ecological land. [Results] The results show that: (1) The proportion of ecological land was the highest among the production-living-ecological land in Yinchuan City, but this proportion decreased during the study period, from 69.49% in 1990 to 57.68% in 2020. During the same period, the production land increased steadily, from 28.54% to 32.52%, and the living land expanded rapidly, from 2.37% to 9.81%. The dynamic degree of living land changed dramatically, but the dynamic degree trends of production land and ecological land gradually became smooth. (2) During the study period, the total amount of ESV increased by 384.2365 million yuan, and the positive effect of ecological land quality improvement on ESV was greater than the negative effect of the expansion of production land and living land on ESV. The contribution of regulating services to ESV was the largest and continued to increase, with the proportion increasing from 82.29% in 1990 to 83.76% in 2020, but the proportion of other services had decreased. The negative supply services are mainly related to the increase of water-consuming crop planting. (3) Urban expansion, energy base construction, and migration into the city are important reasons for the decline in ESV, resulting in lower ESV in the urban area, the Ningdong energy base, and key immigration areas; ecological construction is the main reason for the increase in ESV, with significant increases in areas such as the Bailitan Nature Reserve, the core area of the Yellow River Ecological Belt, and the grape production corridor at the eastern foot of the Helan Mountains. [Conclusion] In future development, it is necessary to improve the efficiency of living land and production land use to reduce the pressure of their expansion on ecological land, and to continuously improve the quality of ecological land to promote the healthy and sustainable development of the desert oasis ecosystem.